別讓樣本征服了您的實驗!!
新一代的 SuperScript® IV Reverse Transcriptase 具備了
◉ 對 RNA 樣本中的抑制物具高抗性
◉ 更高的熱穩定性(可達55℃)
◉ 適用的 RNA 樣本來源更廣
◉ 反應時間更快，只需10分鐘
RNA 樣本中的抑制物對 SuperScript® IV Reverse Transcriptase 影響小
RNA sample中的抑制物
| Inhibitor | Source |
|---|---|
| Ethanol/isopropanol, Salts, Phenol/chloroform, Detergents | Sample prep |
| Heparin, Hematin, Bile salts | Blood, Feces |
| Humic acid, Polyphenols, Polysaccharides | Soil, Plants |
| Formalin, Paraffin | FFPE |
No
inhibitors
Biological inhibitors
0.01 U/ul Heparin
Sample Prep inhibitors
5% Ethanol
Figure1. Analysis of first-strand cDNA synthesis performed in the presence of both biological and sample prep inhibitors.
針對降解的RNA樣本，以及難反轉錄的RNA樣本來源，具有高敏感性和cDNA合成可重複性的優勢。
Figure2. Higher reverse transcription efficiency with degraded RNA using SuperScript® IV RT.
最低只需10分鐘即可合成cDNA，且不影響產率。
Figure3. Fast cDNA synthesis rate in just 10 minutes.
Figure4. The cDNA synthesis protocol for SuperScript® IV Reverse Transcriptase
訂購資訊
| 品名 | 包裝 | 編號 |
|---|---|---|
| SuperScript® IV Reverse Transcriptase | 2,000 units | 18090010 |
| SuperScript® IV Reverse Transcriptase | 10,000 units | 18090050 |
| SuperScript® IV First-Strand Synthesis System | 50 rxns | 18091050 |
| SuperScript® IV First-Strand Synthesis System | 200 rxns | 18091200 |
獲得更多訊息可詢問量子生物科技區域業務專員。